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Table 1 Antibacterial Bioassay of different extracts of leaves of Combretum album and susceptibility test of some standard antibiotics

From: Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria

Bacterial strains Diameter of inhibition zone(mm) Antibiotics a
  Crude extract Cold aqueous extract (μg/ml) Hot aqueous extract (μg/ml) Ethanol extract (μg/ml) Distilled water DMSO Ampicillin Gentamycin Penicillin G Erythromycin Tetracycline Amoxicillin
  0.1% 50 100 200 400 50 100 200 400 50 100 200 400 100 μl/well 100 μl/well (10 μgdisc) (30 μg/disc) (10 μg/disc) (5 μg/dics) (10 μg/disc) (30 μg/disc)
Human pathogens 100 μl/well       
B. subtilis (MTCC 441) 20.33 ± 0.33 21.33 ± 0.33 23.00 ± 0.58 24.33 ± 0.33 26.00 ± 0.58 18.00 ± 0.58 18.67 ± 0.33 19.67 ± 0.33 22.67 ± 0.33 23.67 ± 0.33 24.33 ± 0.33 25.67 ± 0.33 27.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 NA 0.00 ± 0.00 29.67 ± 0.33 29.00 ± 0.00 15.33 ± 0.33
E. coli (MTCC 739) 21.00 ± 0.00 16.33 ± 0.33 17.00 ± 0.00 18.33 ± 0.33 21.67 ± 0.88 16.67 ± 0.33 17.67 ± 0.33 18.33 ± 0.33 22.33 ± 0.67 17.33 ± 0.67 20.00 ± 0.58 21.67 ± 0.33 23.67 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 NA 23.67 ± 0.88 NA 23.00 ± 0.58 24.00 ± 0.00 NA
P. aeruginosa (MTCC 2453) 22.00 ± 0.58 16.67 ± 0.33 17.67 ± 0.67 19.33 ± 0.33 21.00 ± 0.58 16.33 ± 0.33 17.33 ± 0.67 18.67 ± 0.33 20.33 ± 0.33 20.00 ± 0.58 22.33 ± 0.33 24.33 ± 0.33 27.33 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 NA 27.33 ± 0.67 NA 23.33 ± 0.67 14.33 ± 0.67 NA
Fish pathogens        
B. licheniformis (MTCC 530) 22.33 ± 0.33 17.67 ± 0.33 19.00 ± 0.00 19.67 ± 0.33 21.00 ± 0.00 18.67 ± 0.33 19.00 ± 0.00 20.33 ± 0.33 21.00 ± 0.58 22.00 ± 0.58 22.33 ± 0.33 25.33 ± 0.33 27.67 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 NA 0.00 ± 0.00 18.66 ± 0.33 22.00 ± 0.58 17.33 ± 0.33
B. mycoides (MTCC 7343) 21.33 ± 0.33 17.67 ± 0.33 20.33 ± 0.33 21.00 ± 0.58 22.33 ± 0.33 16.67 ± 0.33 18.33 ± 0.33 19.67 ± 0.33 22.00 ± 0.58 19.33 ± 0.67 20.67 ± 0.33 22.00 ± 0.58 24.67 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 NA 0.00 ± 0.00 26.00 ± 0.58 15.00 ± 0.00 12.00 ± 0.58
P. putida (MTCC 1654) 16.67 ± 0.33 16.00 ± 0.58 17.00 ± 0.58 18.00 ± 0.58 20.33 ± 0.33 16.67 ± 0.33 17.67 ± 0.33 19.00 ± 0.58 20.00 ± 0.58 16.33 ± 0.33 18.00 ± 0.00 19.67 ± 0.33 21.33 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 NA 26.67 ± 0.33 NA 25.33 ± 0.33 14.33 ± 0.33 NA
P. fluoroscens (MTCC 103) 22.00 ± 0.00 17.33 ± 0.67 19.67 ± 0.33 21.67 ± 0.67 23.33 ± 0.33 21.00 ± 0.58 22.33 ± 0.67 23.00 ± 0.58 24.00 ± 0.58 21.33 ± 0.33 23.33 ± 0.33 25.00 ± 0.00 26.67 ± 0.33 0.00 ± 0.00 0.00 ± 0.00 NA 23.67 ± 0.33 NA 29.67 ± 0.33 14.00 ± 0.88 NA
  1. a Antibiotics were used based on their spectrum of activity i.e., their efficacy against gram positive, gram negative or both