Fig. 3

Anti gastro-ulcerogenic effect of P. racemosa leaves AME. a A photomicrograph of gastric tissue for negative control group, showed normal stomach tissue which is divided histologically into mucosal layer formed of straight tubular glands (thin arrow) then lined by the sub mucosa (thick arrow) and the connective tissue of the sub mucosa (*) finally the smooth muscle layer (S.M) (H&E X 100). b Higher magnification of (a) showed, the chief cells (thin arrow) from which mainly the glands formed with scattered parietal cells known by its eosinophilic cytoplasm (thick arrow) (H&E X 200). c A photomicrograph of gastric tissue for positive control (ethanol group), showed ulcerated stomach tissue with loss of most of the mucosal glands (*) (H&E X40). d Higher magnification of the (c) showed the area of ulceration with disrupted glands (thin arrow) and others are atrophied (thick arrow) (H&E X 200). e A photomicrograph of gastric tissue for ranitidine 50 mg/kg b.wt. treated group showed slight ulcerated and distorted mucous membrane (H&E X100). f A photomicrograph for of gastric tissue in rats treated with AME of P. racemosa leaves (125 mg/kg b.wt.) showed the ulcerated area with losing the mucosal glands (thick arrow) (H&E X 100). g Higher magnifications of (f), showed sever destruction of the mucosal glands (thin arrow) (H&E X 200). h A photomicrograph for of gastric tissue in rats treated with AME of P. racemosa leaves (250 mg/kg) showed the ulcerated area without healing features (*)(H&E X 100). i Higher magnification of (h), showed exaggerated parietal cells with its eosinophilic cytoplasm (thin arrow) (H&E X 200). j A photomicrograph for of gastric tissue in rats treated with AME of P. racemosa leaves (500 mg/kg b.wt.) showed ulcerated area started to be healed (*) by formation of the granulation tissue (thick arrow) (H&E X 100). k Higher magnification of (j). showed the granulation tissue formed of connective tissue fibers and minute blood vessels formed, beneath this tissue there are gradual revolution of the glands (thin arrow) (H&E X 200)