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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Ethanol extracts from the branch of Taxillus yadoriki parasitic to Neolitsea sericea induces cyclin D1 proteasomal degradation through cyclin D1 nuclear export

Fig. 6

Cyclin D1 degradation by TY-NS-B is dependent on T286 phosphorylation. HCT116 and SW480 cells were transfected with HA-tagged wild type-cyclin D1 or T286A-cyclin D1, and then co-treated with TY-NS-B (50 μg/ml) for the indicated times. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against HA-cyclin D1. Actin was used as internal control for Western blot analysis. Relative density for Western blot was measured using the software Un-SCAN-IT gel Version 5.1 (Silk Scientific, Inc). Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B

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