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Table 3 Group names and definition of the conditions used to treat the various experimental groups of cells or animals. In vivo Western blotting/ELISA experimenta

From: Dendrobium nobile Lindley and its bibenzyl component moscatilin are able to protect retinal cells from ischemia/hypoxia by dowregulating placental growth factor and upregulating Norrie disease protein

Group names Definition of conditions of animals that received
i. Sham (n = 10; control) sham procedure
ii. Vehicle+I/R (n = 10) Pre-ischemic oral vehicle followed by I/R
iii. DNL1.0 + I/R (n = 10) Pre-ischemic oral DNL1.0 followed by I/R
iv. JIB-04 + I/R (n = 4) Pre-ischemic intravitreous JIB-04 followed by I/R
v. Shikonin+I/R (n = 7) Pre-ischemic intravitreous shikonin followed by I/R
vi. Avastin+I/R (n = 4) Pre-ischemic intravitreous avastin followed by I/R
vii. Eylea+I/R (n = 4) Pre-ischemic intravitreous eylea followed by I/R
  1. aThese animals were evaluated by Western blotting/ELISA assays and sacrificed for the measurement of various proteins, namely HIF-1α, VEGF, PKM2, RBP2 and PLGF. Subtotally, 49 animals were used. The number of animals utilized for various defined procedures was 120 (=68 + 49 + 3) and this included animals (n = 3) that died during the retinal ischemia. Pre-ischemia intravitreous injection of 1.0 g/kg/day of DNL (DNL1.0 + I/R), or inhibitors/antibodies of PKM2 (shikonin), RBP2 (JIB-04), VEGF-A (avastin), PLGF (Eylea), or the same volume of vehicle was administered. Abbreviations: HIF-1α hypoxia inducible factor, VEGF vascular endothelium growth factor, PKM2 pyruvate kinase M2, RBP2 retinoblastoma-binding protein 2, PLGF placental growth factor