Fig. 1

a~e The cell viability study of the effects of moscatilin on RGC-5 cells subjected to OGD using light microscopy. After pre-administration of DMSO (vehicle) followed by OGD (DMSO+OGD), the cells were found to be less numerous and some were deformed (as indicated by arrows) as compared to the normal control group (cells cultured in DMEM and pre-administration of DMSO; DMSO+DMEM). These OGD-induced alterations were mitigated by pre-administration of moscatilin (Mos) at 1 h pre-OGD (Pre-OGD 0.1 μM Mos). f The effects of moscatilin on cells subjected to OGD analyzed quantitatively by MTT assay. ** indicates a significant difference (P < 0.01) between the control (DMSO+DMEM) and the “DMSO+OGD” group. † indicates a significant difference (P = 0.04) between the “DMSO+OGD” group and the “Pre-OGD 0.1 μM Mos” group. Please refer to Table 1 for definitions of the other two groups, namely “During OGD 0.1 μM Mos” and “Post-OGD 0.1 μM Mos”. Results are presented as means±S.E.M. (n = 6). DMSO, dimethyl sulfoxide; DMEM, Dulbecco’s modified Eagle’s medium; OGD, oxygen glucose deprivation; RGC, retinal ganglion cell; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Scale = 50 μm