Fig. 4

The effects of Zyflamend and an inhibitor of DAPK on the phosphorylation of CaMKK2 (at Ser511), in CWR22Rv1 cells. A Western blot of pCaMKK2 ± Zyflamend (200 μg/mL, 30 min) and following treatment of DAPKi (20 μM, 1 h – 24 h) followed by the treatment of Zyflamend (200 μg/mL, 30 min). B, C Western blot and graph comparison of pCaMKK2 following treatment of DAPKi (20 μM, 24 h) ± Zyflamend (200 μg/mL, 30 min). (D, E) Western blot and graph comparison of pAMPK following treatment of Zyflamend (200 μg/mL, 30 min) and DAPKi (20 μM, 24 h) ± Zyflamend (200 μg/mL, 30 min). Data are presented as mean ± SEM, n = 3. Bars with different letters are statistically different at p < 0.05. Abbreviations: CON, Control; DAPKi, DAPK inhibitor; Zyf, Zyflamend