Fig. 4

Effect of Cucumis sativus L. extract on LPS-induced angiogenesis. pAECs were cultured on a extracellular matrix with LPS (10 μg/ml) in the absence (a) or presence of increasing doses (0.02; 0.2; 2 mg/ml) of Cucumis sativus L. extract (b, c, d respectively) for 18 h. LPS induced a capillary like network (a, e) while CSE reduced LPS-induced angiogenesis at any doses tested (b-e). Data shown are representative of at least three independent experiments and represent the mean ± SEM. Different letters above the bars indicate significant differences (p < 0.05 ANOVA post hoc Tukey’s test)