Table 2 FTIR band assignments for functional groups found in the second derivative spectra of 3T3-L1 cells [27, 28]
From: Oroxylum indicum (L.) Kurz extract inhibits adipogenesis and lipase activity in vitro
2nd derivative spectra (cm− 1) | Band assignment |
|---|---|
2962 | Asymmetrical stretching (C-H) from methyl (-CH3) groups of lipids |
2924 | Asymmetrical stretching (C-H) from methylene (-CH2) groups of lipids |
2854 | Symmetrical stretching (C-H) from methylene (-CH2) groups of lipids |
1740 | (C=O) of ester functional groups primarily from lipid and fatty acids |
1647 | Amide I protein (C=O stretching) |
1543 | Amide II protein (N-H bending, C-H stretching) |
1462 | Asymmetrical deformation (CH2)scissor from methylene (-CH2) groups of lipids |
1400 | Symmetrical stretching (COO−) associated with symmetrical in-plane deformation bend (CH3) of proteins |
1234 | Asymmetrical stretching (PO2−) mainly nucleic acids with the little contribution from phospholipids |
1153 | Asymmetrical stretching (CO-O-C) of glycogen, other carbohydrates and nucleic acids |
1080 | Symmetrical stretching (PO2−) of the phosphodiester backbone of nucleic acids (DNA and RNA) and phospholipids |
1018 | (C-O) vibration from glycogen and other carbohydrates |