Fig. 4

Microscopic imaging of intracellular lipid after Oil Red O and heamatoxylin staining of the samples. 3T3-L1 cells are stained with Oil Red O and heamatoxilin. The lipid droplets were red in appearance and the nuclei were blue in colour. The arrows indicate lipid droplets within the adipocytes. The size and the number of lipid droplets appear to be a larger in untreated adipocytes (b and c). a non-differentiated cells (pre-adipocytes); b differentiated cells (untreated adipocytes); c vehicle control; d, e, f and g differentiated cells treated with Oroxylum indicum extract at the dose 50 μg/mL, 100 μg/mL, 150 and 200 μg/mL, respectively; h simvastatin at 1.67 μg/mL (original magnification at ×40, scale bar; 100 μm and Inset view at × 400, scale bar; 50 μm)