Fig. 3From: Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating propertiesEffect of propolis extracts on mitochondrial superoxide generation in C6 cells. C6 cells were pre-treated with MitoTracker Green FM (60 nM) for 45 min and with MitoSox Red (5 μM) for 15 min. After incubation, cells were treated with different concentrations (1–15 μg/ml) of AqEP, Pg-AqEP and EEP and incubated for 15 min. Solvent only-treated cells served as controls. Antimycin A (20 μM) treated cells were used as positive control. Images were acquired under fluorescence microscope and analyzed by ImageJ freeware. Data are presented as means of percentage of control cells ± SE . * p < 0.05 versus controlBack to article page