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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Homoharringtonine regulates the alternative splicing of Bcl-x and caspase 9 through a protein phosphatase 1-dependent mechanism

Fig. 5

CalyculinA blocks effects of HHT on Bcl-x and Caspase 9 splicing. Cells were pretreated with either 2 nM calyculin A or with 5 nM okadaic acid, and then exposed to the indicated concentration of HHT for 24 h. Semi-quantitative RT-PCR was performed. a Effects of calyculin A on HHT-induced Bcl-x splicing in MCF7 cells. b Effects of calyculin A on HHT-induced Bcl-x splicing in A549 cells. c Effects of calyculin A on HHT-induced Bcl-x splicing in UACC903 cells. d Densitometric analysis of the ratio of Bcl-xL/xS in MCF7, A549 and UACC903 cells (*P < 0.05). e Effects of calyculin A on HHT-induced Caspase 9 splicing in MCF7 cells. f Densitometric analysis of the ratio of Caspase 9a/9b in MCF7 (*P < 0.05). g Effects of calyculin A on HHT-induced Caspase 9 splicing in UACC903 cells. h Densitometric analysis of the ratio of Caspase 9a/9b in UACC903. +, with HHT, okadaic acid or calyculin A; −, without HHT, okadaic acid or calyculin A (*P < 0.05)

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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