Fig. 2

HHT regulates Bcl-x and Caspase 9 splicing in MCF7 cells. MCF7 cells were treated with HHT. Total RNA was extracted and analyzed by semi-quantitative RT-PCR for the alternative splicing of Bcl-x and Caspase 9. a Decrease of Bcl-xL and increase of Bcl-xS is correlated with HHT concentration. b Densitometric analysis of the ratio of Bcl-xL/xS (*P < 0.05 compared to cells treated with control). c and d Cells were treated with 0.5 μM HHT for different durations. Semi-quantitative RT-PCR was performed to quantify Bcl-x splicing (*P < 0.05 compared to cells at 0 h). e Decrease of Caspase 9b and increase of Caspase 9a is correlated with HHT concentration. f Densitometric analysis of the ratio of Caspase 9a/9b (*P < 0.05 compared to cells treated with control). g and h Cells were treated with 0.5 μM HHT for different durations. Semi-quantitative RT-PCR was then performed to quantify the alternative spliced products of Caspase 9 (*P < 0.05 compared to cells at 0 h)