Fig. 8

Biofilm growth at the solid/air interface. E. coli biofilm grown on polycarbonate membrane under three experimental conditions: i) treatment 1: growth in contact with 1 ml of LB with 10 ppm of E. acoroides leaf extract; ii) treatment 2: overnight culture grown with 10 ppm of E. acoroides leaf extract and then growth in contact with 1 ml of LB; iii) treatment 3: overnight culture grown with 10 ppm of E. acoroides leaf extract and growth in contact with 1 ml of LB with 10 ppm of E. acoroides leaf extract. In the positive control, microorganisms grew in 1 ml LB inside a basolateral well without the extract. Data obtained were divided by the area of the membrane, and means were reported. The experiment was repeated three times. (T1 = treatment 1; T2 = treatment 2; T3 = treatment 3; C + = Positive control)