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Table 1 Primers sequences of target genes

From: Curcumin derivative, 2,6-bis(2-fluorobenzylidene)cyclohexanone (MS65) inhibits interleukin-6 production through suppression of NF-κB and MAPK pathways in histamine-induced human keratinocytes cell (HaCaT)

Genes   Primer sequences (5′-3′) Accession number
H1R F
R
CTG GTT TCT CTC TTT TCT GTG GGT T
GAT CTT GGC ATA GAA CCA GAG CAT G
NM_001098213.1
PKC F
R
CAG GCA GAA ATT CGA GAA AGC CAA A
TCC CAA CAC CAT GAG GAA ATT GAA G
NM_002737.2
IKK-β F
R
ATC CCC GAT AAG CCT GCC A
CTT GGG CTC TTG AAG GAT ACA G
NM_001556.2
IκB-α F
R
ATT GCT GAG GCA CTT CTG GGA GCT G
AGA CAC GTG TGG CCA TTG TAG TTG G
NM_020529.2
NF-κβ F
R
GGA CCG CTG CAT CCA CAG TTT CCA G
TGT CAC CTG GAA GCA GAG CCG CAC A
NM_001145138.1
c-Raf F
R
CAG TAT CTG GGA CCC AGG AGA AAA A
TAG GAT CTT TAC TGC AAC ATC TCC G
NM_002880.3
MEK F
R
CGT ACA TCG TGG GCT TCT ATG GTG C
GCA TGA TCT TGT GCT TCT CCC TCA G
NM_002755.3
ERK F
R
CTC TAC CAG ATC CTC AGA GGG TTA A
TAC CAA CGT GTG GCC ACA TAT TCT G
NM_002745.4
GAPDH F
R
CAG CCT CAA GAT CAT CAG CA
CAT CCA CAG TCT TCT GGG TG
NM_002046.5
  1. H1R histamine H1-receptor, PKC protein kinase, IKK-β inhibitor of nuclear factor kappa-B kinase subunit beta, IκB-α Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NF-κβ nuclear factor kappa-light-chain-enhancer of activated B cells, c-Raf RAF proto-oncogene serine/threonine-protein kinase, MEK mitogen-activated protein kinase kinase, ERK extracellular signal–regulated kinases, GAPDH glyceraldehyde 3-phosphate dehydrogenase