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Table 2 Cytotoxicity of the crude extract, aqueous alkaloid-enriched fraction and solamargine as described by their IC50 values in a panel of cancerous and non-cancerous cell lines

From: Solamargine, a bioactive steroidal alkaloid isolated from Solanum aculeastrum induces non-selective cytotoxicity and P-glycoprotein inhibition

  IC50 ± SEM
  Cancerous cell lines Non-cancerous cell lines
Extract/fractiona A2780 Caco-2 DU-145 HepG2 MCF-7 MDA-MB-231 SH-SY5Y SK-Br3 3 T3-L1 C2C12 EA.hy.926 SC-1
Crude 24 ha 34.73 ± 1.07 36.46 ± 1.20 74.33 ± 1.14 9.44 ± 1.11 44.35 ± 1.06 26.87* 19.10 ± 1.07 33.25 ± 1.10 37.24 ± 1.34 93.41 ± 1.08 3.88 ±
1.07
28.73 ± 1.09
Crude 72 ha 32.88* 24.40 ± 1.13 47.11 ± 1.18 7.04 ± 1.08 27.94 ± 1.11 24.00* 12.97 ± 1.07 20.11 ± 1.08 19.55 ± 1.23 91.18 ± 1.13 2.79 ±
1.55
23.07 ± 1.10
Aqueous 24 ha 31.45* 50.37 ± 1.26 46.53 ± 1.21 40.02 ± 1.08 31.91 ± 1.06 28.05* 29.11 ± * 29.24* 21.95 ± 1.50 >  100 16.45
± 1.06
19.89 ± 1.09
Aqueous 72 ha 29.81* 25.21 ± 1.12 34.08* 20.66 ± 1.10 25.49 ± 1.13 26.11* 17.21 ± 1.15 18.81 ± 1.14 12.89 ± 1.23 62.00 ± 1.22 9.35 ±
1.08
21.10 ± 1.08
Solamargine 72 hb X X X X X X 15.62 ± 1.45 18.59 ± 1.13 X 20.25 ± 1.08 8.30 ±
1.12
X
P-gp inhibitor
 Verapamil 24 hb 93.48 ± 1.05 >  100 >  100 18.72 ± 1.08 >  100 >  100 >  100 >  100 >  100 >  100 31.55 ± 1.12 >  100
 Verapamil 72 hb 65.75 ± 1.06 >  100 >  100 8.34 ± 1.08 46.76 ± 1.14 47.5 ± 1.09 >  100 41.92 ± 1.09 79.22 ± 1.21 >  100 5.69 ±
1.11
60.01 ± 1.16
  1. * Ambiguity in data points (GraphPad 5.0 software could not predict SEM due to steepness of dose-response curve)
  2. apresented in μg/mL
  3. Bpresented in μM
  4. XNot tested on mentioned cell line