Table 1 Primer sequences of inducible nitric oxide synthase (iNOS) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) used in the quantitative real time PCR (qRT-PCR) assay. Beta-actin (β-actin), hypoxanthine phosphoribosyltransferase (HPRT) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as housekeeping genes for normalization of the iNOS and NF-kB gene expression
From: Coconut water vinegar ameliorates recovery of acetaminophen induced liver damage in mice
Forward primer (5′-3′) | Reverse primer (3′-5′) | |
|---|---|---|
iNOS | 5′-GCACCGAGATTGGAGTTC-3′ | 3′-GAGCACAGCCACATTGAT-5′ |
NF-κB | 5′-CATTCTGACCTTGCCTATCT-3′ | 3′-CTGCTGTTCTGTCCATTCT-5′ |
β-actin | 5′-TTCCAGCCTTCCTTCTTG-3′ | 3′-GGAGCCAGAGCAGTAATC-5′ |
GAPDH | 5′-GAAGGTGGTGAAGCAGGCATC-3′ | 3′-GAAGGTGGAAGAGTGGGAGTT-5′ |
HPRT | 5′-CGTGATTAGCGATGATGAAC-3′ | 3′-AATGTAATCCAGCAGGTCAG-5′ |