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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Brazilian propolis ethanol extract and its component kaempferol induce myeloid-derived suppressor cells from macrophages of mice in vivo and in vitro

Fig. 5

PEE induces MDSC markers in cultured macrophages. a, b Thioglycolate-elicited peritoneal macrophages, (c, d) J774.1 cells, and (E, F) GM-CSF-elicited M1 and M-CSF-elicited M2 macrophages were stimulated with PEE (a; 1, 10, or 100 μg/mL: b-f; 100 μg/mL) for 24 h. Subsequent qRT-PCR for (a, c, e) Ly6g and (b, d, f) Il10 was performed. Values were normalized to (a-d) Hprt1 mRNA or (e, f) 18 s rRNA levels, and represented as the means ± SD of (a, b, e, f) 4 or (c, d) 3 samples. *P < 0.05 vs. vehicle-treated mice; **P < 0.05 vs. M2 macrophages

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