Fig. 3

Immunohistochemical staining for fibronectin and α-smooth muscle actin (IHC, DAB, ×200). Liver sections from: a Group II showing positive expression of fibronectin in the sinusoids as brownish material (black arrow). b Group I with S. mansoni showing a large fibrocellular bilharzial granuloma positive for fibronectin, and brownish stained collagen bundles (red arrow). Hepatocytes are also shown (black arrow). c Group III showing hepatic tissues positive for fibronectin, stained brownish (black arrow). d Group IV showing small fibrocellular granulomata, positive for fibronectin immunostaining in both granuloma (red arrow) and hepatocytes (black arrow). e Group V showing few hepatocytes positive for fibronectin immunostaining (black arrow). f Group VII with small fibrocellular granulomata, positive for fibronectin in both the granuloma (red arrow) and hepatocytes (black arrow). g Group II showing positive expression of α-smooth muscle actin in the sinusoids as brownish material (black arrow). h Group II showing a large fibrocellular bilharzial granuloma positive for α-smooth muscle actin. Collagen bundles are stained brownish (red arrow) and hepatocytes are also indicated (black). i Group III showing few hepatic cells positive for α-smooth muscle actin, stained brownish (black arrow). j Group IV showing small fibrocellular granulomata positive for α-smooth muscle actin in granuloma (red arrow). Hepatocytes are also shown (black arrow). k Group V showing few hepatocytes positive for α-smooth muscle actin (black arrow). l Group VII showing small fibrocellular granulomata positive for α-smooth muscle actin in the granuloma (red arrow). Hepatocytes are also shown (black arrow)