Fig. 6

Effects of ethanol extract of Coicis Semen (CSE) on the expression of unfolded protein response (UPR)-associated proteins. a Western blot analysis of the levels of UPR-associated proteins (p-PERK, p-eIF2α and ATF6α) after treatment with CSE (200, or 300 μg/mL) for 24 h or 48 hwith or without 1 mM 4-PBA in Chang liver cells, (b) Quantitative graph of the levels of p-PERK, (c) p-eIF2α and (d) ATF6α. Tunicamycin (TM, 2 μg/mL) was used as the positive control and 1 mM 4-PBA was used to inhibit ER stress. Data are presented as the mean ± SEM from at least three independent experiments and were compared with the vehicle control [0.5% dimethyl sulfoxide (DMSO)]. *: p < 0.05, **: p < 0.01, #: Significant difference (p < 0.05) compared with treatment with CSE alone (200 μg/mL).β-Actin was used as the control