Fig. 3

Effects of ethanol extract of Coicis Semen (CSE) and coixol on the gene expression of total XBP1, unspliced XBP1, spliced XBP1, and XBP1 cut by PstI in Chang liver cells. a CSE 200, 300, 400, or 500 μg/mL; b coixol 100, 200, or 300 μg/mL. Data are presented as the mean ± SEM from three independent experiments and were compared with the vehicle control [0.5% dimethyl sulfoxide (DMSO)]. TM: tunicamycin;*: p < 0.05, **: p < 0.01. β-Actin was used as the reference gene to normalize the data using the ΔΔCt method