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Table 2. Characteristics of sequences and derived designed primers for high resolution melting analysis

From: Species identification approach for both raw materials and end products of herbal supplements from Tinospora species

  matK rbcL trnL ITS1
Available species 199 271 142 156
Number of species in analysed data set 178 256 136 98
Characters in an in silico analysis (bp) 158 181 118 315
Variable characters (%) 135/158 (85.44%) 42/181 (23.20%) 36/118 (30.51%) 274/315 (86.98%)
Conserved sites (%) 23/158 (14.56%) 107/181 (59.12%) 73/118 (61.86%) 41/315 (13.02%)
Average %GC content 34.6 43.4 42.5 53.9