Fig. 3
The estrogenic activity of the crude drugs present in UKT. a MtT/Se cells were incubated with each of the crude drugs found in UKT at a concentration of 1 μg/ml (white columns), 5 μg/ml (gray columns), or 10 μg/ml (black columns), and the cell proliferation induced by treatment with these crude drugs or 10− 7 M of E2 for 6 days was evaluated. Cell proliferative activity is shown as a percentage compared with that induced by DMSO alone in the MTT assay. **significantly increased compared with the activity induced by DMSO, p < 0.01. b The β-galactosidase activity of all 12 of the crude drugs present in UKT was evaluated at concentrations of 0.1 mg/ml (white columns) and 0.5 mg/ml (black columns). The data for the crude drugs that did not display significant activity in the yeast two-hybrid assay are not shown. **significantly increased compared with the activity of DMSO, p < 0.01