Fig. 3From: The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining themThe estrogenic activity of the crude drugs present in UKT. a MtT/Se cells were incubated with each of the crude drugs found in UKT at a concentration of 1 μg/ml (white columns), 5 μg/ml (gray columns), or 10 μg/ml (black columns), and the cell proliferation induced by treatment with these crude drugs or 10− 7 M of E2 for 6 days was evaluated. Cell proliferative activity is shown as a percentage compared with that induced by DMSO alone in the MTT assay. **significantly increased compared with the activity induced by DMSO, p < 0.01. b The β-galactosidase activity of all 12 of the crude drugs present in UKT was evaluated at concentrations of 0.1 mg/ml (white columns) and 0.5 mg/ml (black columns). The data for the crude drugs that did not display significant activity in the yeast two-hybrid assay are not shown. **significantly increased compared with the activity of DMSO, p < 0.01Back to article page