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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them

Fig. 1

The cell proliferation activity induced by Japanese herbal medicines. a MtT/Se cells were incubated with 50 μg/ml (white columns) or 100 μg/ml of each Japanese herbal medicine (black columns) or 10− 7 M of E2 for 6 days. Cell proliferative activity is shown as a percentage compared with that induced by DMSO alone in the MTT assay. **significantly increased compared with the activity induced by DMSO, p < 0.01. b Cells were treated with 10 nM ICI alone or a combination of 10 nM ICI and 100 μg/ml of UKT, KSS, or NSS or 10− 7 M E2 for 6 days. White columns: ICI(−)test substance(−); blank columns: ICI(−)test substance(+); gray columns: ICI(+)test substance(−); diagonal columns: ICI(+)test substance(+). **significantly inhibited the induction of cell proliferation compared with herbal medicines or E2 alone, p < 0.01; *p < 0.05. c The cell proliferation induced by metabolized Japanese herbal medicines. The Japanese herbal medicines were treated with the rat S9 fraction, and the proliferative activity induced by each Japanese herbal medicine (100 μg/ml, black columns) was compared with that induced by the unmetabolized medicine (white columns). *significantly increased compared with the activity of the unmetabolized medicine, p < 0.05

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