Fig. 3

In vitro analysis of anticancer activity in A549 cells. a Cytotoxicity analysis in A549 cells identifies IC50 as1.5 mg/ml of GAE. b Fluorescent imaging with Annexin, Propidium iodide and DAPI revealed the translocation of Phosphatidyl serine to the cell membrane and nuclear fragmentation in GAE treated cells. c Confocal imaging of GAE treated cells showing nuclear fragmentation. d FACS analysis of Apoptosis by DAPI staining shows the emergence of apoptotic peak after 6 h of GAE treatment. All experiments were done in triplicate. The values represent Mean ± SD. C-control, T-treated