Inhibition of lipopolysaccharide (LPS)-induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C.D.C.) stem bark, Cryptolepis sanguinolenta (Lindl.) Schltr and Cymbopogon citratus Stapf leaves in raw 264.7 macrophages and U87 glioblastoma cells

Table 1 Details of primer sequences (Sigma Genosys) used for inflammatory mediators gene expression through RT-PCR amplification

Genes	Oligoname	Oligonucleotide sequence 5′…………… ………………..3′	Length	Anneal T°
IL6	Human-IL6-FP	TTCGGTCCAGTTGCCTTCTC	20	61.8 °C
IL6	Human-IL6-RP	GAGGTGAGTGGCTGTCTGTG	20	61.8 °C
TNFα	Human-TNFalpha-FP	CTCCAGGCGGTGCCTTGTTC	20	60.4 °C
TNFα	H-TNFalpha-RP	CAGGCAGAAGAGCGTGGTG	19	60.4 °C
IL1β	Human-IL1beta-FP	GCAAGGGCTTCAGGCAGGCCGCG	23	64 °C
IL1β	Human-IL1beta-RP	GGTCATTCTCCTGGAAGGTCTGTGGGC	27	64 °C
NF-kB	Human-Nf-kB-FP	GCGCTTCTCTGCCTTCCTTA	20	58 °C
NF-kB	Human-Nf-kB-RP	TCTTCAGGTTTGATGCCCCC	20	58 °C
GAPDH	Human-GAPDH-FP	ACCACAGTCCATGCCATCAC	20	60.4 °C
GAPDH	Human-GAPDH-RP	TCCACCACCCTGTTGCTGT	19	60.4 °C

ISSN: 2662-7671