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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Plumbagin induces RPE cell cycle arrest and apoptosis via p38 MARK and PI3K/AKT/mTOR signaling pathways in PVR

Fig. 5

Effect of plumbagin on the different signal pathways in ARPE cells. (*:P < 0.05 by one-way ANOVA, n = 3). Notes: (a) The relative mRNA expression levels of p38 MARK, PI3K, β-catenin and Notch-1 determined by QPCR when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM. β-actin was used as the internal control; (b) The relative mRNA expression levels of p38 MARK and PI3K determined by QPCR when ARPE cells were treated with plumbagin at 15 μM for 0, 12, 24 and 48 h. β-actin was used as the internal control; (c) The protein levels of p-p38 MARK, p-PI3K, β-catenin and Notch-1 determined by Western blotting assay. GAPDH was used as the internal control; (d) The relative protein levels of p-p38 MARK, p-PI3K, β-catenin and Notch-1 when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM; (e) The relative protein levels of p-p38 MARK, p-PI3K, β-catenin and Notch-1 when ARPE cells were treated with plumbagin at 15 μM for 0, 12, 24 and 48 h

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