Fig. 4

Effect of plumbagin on the expression of apoptosis-related proteins in ARPE cells. (*:P < 0.05 by one-way ANOVA,n = 3). Notes: (a) The expression levels of Bax, Bak, Bcl-2 and Bcl-xl determined by Western blotting assay when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM. β-actin was used as the internal control. b Bar graphs show the expression levels of Bax and Bak when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM for 24 h. c Bar graphs show the expression levels of Bcl-2 and Bcl-xl when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM for 24 h. d Bar graphs show the expression levels of Bax and Bak when ARPE cells were treated with plumbagin at 15 μM for 0, 12, 24 and 48 h. e Bar graphs show the expression levels of Bcl-2 and Bcl-xl when ARPE cells were treated with plumbagin at 15 μM for 0, 12, 24 and 48 h. f The expression levels of Bax, Cytochrome C, Caspase-3 and Caspase-8 determined by ELISA when ARPE cells were treated with plumbagin at 0,5,15 and 25 μM for 48 h