Fig. 2

Plumbagin enhanced apoptosis of ARPE cells. The apoptosis of cells was analyzed by Annexin V-FITC/PI assay. Data were represented the mean ± SD of three individual experiments. (*P < 0.05 by one-way ANOVA.). Notes: (a) Flow cytometric plots show cells in the live, early apoptosis, and late apoptosis stages when the cells were treated with PLB at 0, 5, 15 and 25 μM for 24 h; (b) Bar graphs show the percentage of specific cell populations (live, early apoptosis, or late apoptosis) and total apoptosis cell population when the cells were treated with PLB at 0, 5, 15, and 25 μM for 24 h; (c) Flow cytometric plots show cells in the live, early apoptosis, and late apoptosis stages when the cells were treated with 15 μM PLB at 0, 12, 24,and 48 h; (d) Bar graphs show the percentage of specific cell populations (live, early apoptosis, or late apoptosis) and total apoptosis cell population when the cells were treated with 15 μM PLB at 0, 12, 24, and 48 h