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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: A Chinese herbal decoction, Jian-Pi-Yi-Shen, regulates the expressions of erythropoietin and pro-inflammatory cytokines in cultured cells

Fig. 2

JPYS induces HRE-mediated transcriptional activity. A luciferase-reporter containing six HREs and a downstream luciferase-reporter gene, namely as pHRE-Luc, was used as a study tool (upper panel). Cultured HEK293T cells, transfected with pHRE-Luc, were treated with JPYS extracts (1–30 μg/ml) for 24 h. The cell lysates were subjected to luciferase assay. The mineral oil layering (oil: medium = 2: 1) served as a positive control, with the response about 70% of increase. Values are expressed as the fold of increase to basal reading (untreated culture), and they are in Mean ± SD, where n = 3, each with triplicate samples. Statistical comparison was made with the control; * p < 0.05; ** p < 0.01; *** p < 0.001

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