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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Immune-enhancing activity of C. militaris fermented with Pediococcus pentosaceus (GRC-ON89A) in CY-induced immunosuppressed model

Fig. 5

The effect of GRC-ON89A on the phagocytosis related signaling molecules and on the levels of cytokine mRNA expression. a GRC-ON89A activated FcγR signaling pathway. RAW264.7 cells (1 × 106 cells/ml) were treated with GRC-ON89A for 1 h. b Total protein lysates were analyzed by Western blot analysis for phosphorylated – and total forms of Lyn, Syk, ERK, p38, JNK, NFκB, and IκB. β-Actin was used as the internal control. c The expression level of IL-10 and TNF-α mRNA in RAW 264.7 cells was determined by RT-PCR. Each figure is representative of three independent experiments. Data comparisons between groups were analyzed using Student’s t-test and One-way ANOVA with Dunnett’s t-test for significance of individual comparisons (* = p < 0.05, ** = p < 0.01, *** = p < 0.001 vs. control group)

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