Fig. 2

Effects of GRC-ON89A on phagocytic activity of RAW 264.7 cells and primary cultured peritoneal macrophages. The phagocytosis activity of 1% GRC-ON89A-treated macrophages was examined in the presence of IgG-opsonized FITC particles for 48 h. a The phagocytic activity of RAW 264.7 macrophages and (b) primary peritoneal macrophages. Real-time cell images were taken and analyzed using Metamorph software (magnification, 100×). The phagocytic index was quantified for statistics analysis using Image J software. Data comparisons among groups were analyzed using Student’s t-test and One-way ANOVA with Dunnett’s t-test for significance of individual comparisons (# = p < 0.05 vs. non-treated control group, ** = p < 0.01, *** = p < 0.001 vs. GRC). c Primary peritoneal macrophag e viability was assessed by CCK-8 assay. Data are represented as the mean ± SD (n > 3) or ± SEM (n > 10). BF denotes bright field. Each figure is representative of three independent experiments; Scale bars = 50 μm