Fig. 2

Transmission electron microscopy of SKOV3 cells treated with fisetin (300 μm) for 24 h. Images were recorded at 5000 × magnification using a transmission electron microscope. a (a) Normal structure of cancer cells. (b) Heterochromatin aggregation in the cell. (c) Structural changes before apoptosis. (d) Chromatin edge set and nuclear membrane thick dyeing. (e) Apoptosis cells. Membrane blebbing appeared inside the cells. (f) Apoptosis cells with apoptotic bodies, swelling of organelles. b-c SKOV3 cells were treated with different concentrations of fisetin/fisetin micelles and labeled with TUNEL and DAPI after 24 h. Images of random fields (n = 10) per slide (n = 3) were collected at 400×. d-e Quantitative analysis of the apoptosis (TUNEL) he apoptotic cells were counted with at least 100 cells from four randomly selected fields in each group. Compared with control, fisetin/fisetin micelles treated SKOV3 cells showed obvious apoptosis in concentration-dependent manner. Representation figures of treated cells labeled for TUNEL and counterstained with DAPI are shown. Scale bar,5um