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Table 2 Extraction yields using different solvents and their inhibition of the elastase and collagenase enzymes

From: Anti-aging potential of extracts from Sclerocarya birrea (A. Rich.) Hochst and its chemical profiling by UPLC-Q-TOF-MS

Extraction solvent used for the stems Extraction yield (calculated from dry plant material) as w/w % Elastase assay (% Inhibition ± SD) Collagenase assay (% Inhibition ± SD)
Acetone 3.36 ** 91.25 ± 0.11 91.17 ± 3.59
Methanol: DCM (1:1) 5.39 ** 89.74 ± 0.32 93.75 ± 3.32
Ethanol 3.09 ** 93.35 ± 0.89 99.08 ± 3.57
Sequential extraction with hexane 0.38 ** 0 ** 11.14 ± 2.51
Sequential extraction with DCM 0.22 ** 6.69 ± 1.36 ** 19.62 ± 2.78
Sequential extraction with ethyl acetate 0.87 ** 65.02 ± 1.57 95.56 ± 1.68
Sequential extraction with methanol 8.01 99.19 ± 0.18 94.46 ± 2.31
   Controls  
10% MeOH   3.28 ± 0.34 0
10% DMSO   4.70 ± 0.53 1.58 ± 0.60
Elafin   99.21 ± 0.05  
EDTA    95.66 ± 2.11
  1. ** showing a significant difference of p < 0.01 with EDTA
  2. ** showing a significant difference of p < 0.01 with elafin