Genotoxicity evaluation of So-ochim-tang-gamibang (SOCG), a herbal medicine

Table 1 Results of the bacterial reverse mutation test for SOCG

	TA98	TA100	TA1535	TA1537	WP2uvrA (pKM101)
Dose (μg/plate)	Number of revertant colonies/plate (in the absence of metabolic activation)^d [factor]^e
Negative control^a	19 ± 3	52 ± 4	6 ± 2	5 ± 2	86 ± 4
313	20 ± 1[1.05]	56 ± 13[1.08]	7 ± 2[1.17]	4 ± 2[0.80]	90 ± 12[1.05]
625	19 ± 4[1.00]	50 ± 7[0.96]	6 ± 2[1.00]	6 ± 3[1.20]	92 ± 6[1.07]
1250	19 ± 4[1.00]	49 ± 7[0.94]	6 ± 2[1.00]	6 ± 1[1.20]	100 ± 5[1.16]
2500	20 ± 3[1.05]	41 ± 8[0.79]	6 ± 1[1.00]	5 ± 1[1.00]	103 ± 6[1.20]
5000	19 ± 3[1.00]	28 ± 3[0.54]	6 ± 1[1.00]	4 ± 1[0.80]	107 ± 11[1.24]
Positive control^b	475 ± 80[25.0]	549 ± 29[10.6]	461 ± 28[76.8]	430 ± 160[86.0]	1056 ± 117[12.3]
Dose (μg/plate)	Number of revertant colonies/plate (in the presence of metabolic activation)^d [factor]^e
Negative control^a	22 ± 3	79 ± 6	9 ± 2	13 ± 2	114 ± 15
313	21 ± 3[0.95]	73 ± 7[0.92]	10 ± 3[1.11]	14 ± 3[1.08]	113 ± 8[0.99]
625	23 ± 3[1.05]	76 ± 4[0.96]	10 ± 2[1.11]	17 ± 3[1.31]	127 ± 17[1.11]
1250	22 ± 2[1.00]	76 ± 10[0.96]	9 ± 2[1.00]	13 ± 4[1.00]	141 ± 7[1.24]
2500	21 ± 5[0.95]	72 ± 10[0.91]	11 ± 4[1.22]	15 ± 3[1.15]	147 ± 9[12.9]
5000	20 ± 6[0.91]	75 ± 4[0.95]	10 ± 3[1.11]	13 ± 1[1.00]	146 ± 6[1.28]
Positive control^c	221 ± 10[10.0]	404 ± 4[5.11]	121 ± 18[13.4]	134 ± 9[10.3]	460 ± 28[4.04]

^aWater for injection
^bTA98: 2-Nitrofluorene (2-MF), 5.0 μg/plate; TA100: Sodium azide (SA), 1.5 μg/plate; TA1535: SA, 1.5 μg/plate; TA1537: 9-Aminoacridine (9-AA), 80 μg/plate; WP2uvrA(pKM101): 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide(AF2), 0.005 μg/plate
^cTA98: 2-Aminoanthracene (2-AA), 1.0 μg/plate; TA100: 2-AA, 2.0 μg/plate; TA1535: 2-AA, 3.0 μg/plate; TA1537: 2-AA, 3.0 μg/plate; WP2uvrA(pKM101): 2-AA, 2.0 μg/plate
^dData expressed as mean ± standard deviation
^eNo. of revertant colonies per treated plate/No. of revertant colonies per negative control plate

ISSN: 2662-7671