Fig. 5
From: Gigantol inhibits Wnt/β-catenin signaling and exhibits anticancer activity in breast cancer cells
Gigantol reduces the mRNA levels of Wnt target genes in breast cancer cells. a-d MDA-MB-231 (a and b) and MDA-MB-468 (c and d) cells were treated with the specified amounts of gigantol for 24 h. RNA was extracted and then reverse-transcribed into cDNA. Prepared cDNA was then subjected to real-time PCR analysis to detect the mRNA expression of Axin2 (a and c) and Survivin (b and d). Gigantol treatment significantly reduced the mRNA level of Axin2 and Survivin compared with DMSO treated control cells, * p < 0.05