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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Cytotoxic activity of the twigs of Cinnamomum cassia through the suppression of cell proliferation and the induction of apoptosis in human colorectal cancer cells

Fig. 5

Contribution of ROS-dependent NF-κB activation to TC-HW-mediated apoptosis. a and bHCT116 and SW480 were co-transfected with NF-κB luciferase construct and pRL-null. The cells were treated with TC-HW in presence/absence of NAC. Luciferase activity for NF-κB was measured as a ratio of firefly luciferase signal/renilla luciferase signal using a dual luciferase assay kit. *P < 0.05 compared to cell without TC-HW. c HCT116 cells were treated with TC-HW (100 μg/ml) in presence/absence of NAC. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against IκB-α. Actin was used as internal control for Western blot analysis. *P < 0.05 compared to cell without TC-HW. d HCT116 cells were treated with TC-HW (100 μg/ml) in presence/absence of NAC. Cytosol and nucleus extracts were prepared and subsequently Western blot analysis was performed for p65. Actin was used as internal control for Western blot analysis. *P < 0.05 compared to cell without TC-HW. e HCT116 cells were transfected with control and p65 siRNA and then treated with TC-HW (100 μg/ml). Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against p65 or cleaved PARP. Actin was used as internal control for Western blot analysis

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