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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Cytotoxic activity of the twigs of Cinnamomum cassia through the suppression of cell proliferation and the induction of apoptosis in human colorectal cancer cells

Fig. 4

Induction of apoptosis by TC-HW through ROS-dependent DNA damage. a HCT116 and SW480 were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cleaved PARP. Actin was used as internal control for Western blot analysis. b HCT116 and SW480 cells were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. Intracellular ROS were measured using OxiSelect™ Intracellular ROS Assay Kit. *P < 0.05 compared to cell without TC-HW. c HCT116 and SW480 were plated overnight and then treated with TC-HW at the indicated concentrations for 24 h. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against phospho-H2AX. Actin was used as internal control for Western blot analysis. d HCT116 and SW480 were pretreated with NAC (10 mM) and then co-treated with TC-HW at the indicated concentrations. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cleaved PARP. Actin was used as internal control for Western blot analysis. e HCT116 and SW480 were pretreated with NAC (10 mM) and then co-treated with TC-HW (100 μg/ml). Cell viability was measured using MTT assay. *P < 0.05 compared to cell without TC-HW. f HCT116 and SW480 were pretreated with NAC (10 mM) and then co-treated with TC-HW (100 μg/ml). Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against phospho-H2AX. Actin was used as internal control for Western blot analysis

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