Fig. 5

Inhibition of LPS-induced nuclear factor-kappa B (NF-κB) protein expression by PS + CR. (a, b) Levels of I-κBα and phospho-I-κBα. RAW 264.7 cells were treated with 100 and 300 μg/ml of PS + CR for 1 h prior to the addition of lipopolysaccharide (LPS) (1 μg/ml) and further incubated for 30 min. Cytosol fraction proteins were separated by SDS-PAGE. β-Actin was used as a loading control, and the bar chart shows the quantitative evaluation of I-κBα and phospho-I-κBα bands by densitometry. (c) The levels of NF-κB. RAW 264.7 cells were treated with 100 and 300 μg/ml of PS + CR for 1 h prior to the addition of LPS (1 μg/ml) and further incubated for 1 h. Nuclear fraction proteins were separated by SDS-PAGE. Lamin A/C was used as a loading control, and the bar chart shows the quantitative evaluation of NF-κB bands by densitometry. Values represent the mean ± S.D. of three independent experiments (significant compared to the control; ^**p < 0.01, significant compared to LPS alone, ^#p < 0.05 or ^##p < 0.01)