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Table 4 MIC of different antibiotics after the association with 3′,4′,7-Trihydroxyflavone MIC/2, MIC/4 against five MDR bacteria strains

From: In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′,4′,7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria

Antibiotics Bacterial strains, MIC (μg/mL) of antibiotics in the absence and presence of 3′,4′,7-Trihydroxyflavone
Compounds concentration PA124 AG100Atet EA289 NEA16 KP63 PBSS (%)
CHL 0 128 8 128 256 256  
MIC/2 16(0.125) S 4(0.5) S 128(1)I 256(1)I 256(1)I 40
MIC/4 32(0.25) S 8(1)I 128(1)I 256(1)I 256(1)I 20
CIP 0 64 8 > 64 > 64 > 64  
MIC/2 32(0.5) S 4(0.5) S > 64(≥ 1)I > 64(≥ 1)I 32(˂0.5) S 60
MIC/4 64(1)I 8(1)I > 64(≥ 1)I > 64(≥ 1)I > 64(≥ 1)I 00
TET 0 16 16 32 64 4  
MIC/2 4(0.25) S < 1(< 0.062) S 64(2)I 32(0.5) S < 0.5(< 0.125) S 80
MIC/4 8(0.5) S < 1(< 0.062) S 64(2)I 32(0.5) S < 0.5(< 0.125) S 80
ERY 0 128 8 128 256 8  
MIC/2 16(0.125) S 4(0.5) S 16(0.5) S 128(0.5) S 8(1)I 80
MIC/4 16(0.125) S 8(1)I 128(1)I 128(0.5) S 8(1)I 40
KAN 0 128 4 8 32 32  
MIC/2 32(0.25) S 4(1)I 4(0.5) S 32(1)I 16(0.5) S 60
MIC/4 64(0.5) S 4(1)I 8(1)I 32(1)I 16(0.5) S 40
STR 0 256 > 256 > 256 128 8  
MIC/2 16(0.0625) S 256(< 1) S > 256(≥ 1)I 128(1)I 4(0.5) S 60
MIC/4 32(0.125) S > 256(≥ 1)I > 256(≥ 1)I 128(1)I 8(1)I 20
  1. aAntibotics [TET tetracycline, CIP ciprofloxacin, STR streptomycin, KAN kanamycin, CHL chloramphenicol, ERY erythromycin]
  2. bBacterial strains: Escherichia coli [AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [EA289], Klebsiella pneumonia [KP63], Providencia stuartii [NAE16]
  3. cPBSS percentage of bacteria strain on which synergism has been observed, (): FIC (Fractional Inhibitory Concentration) of the antibiotics after association with compounds, S Synergy, I Indifference; The values in bold represent the cases of synergy between extract and antibiotic