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Table 3 MIC of different antibiotics after the association of the crude MFS extract at MIC/2, MIC/4 against ten MDR bacteria strains

From: In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′,4′,7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria

Antibiotics

Bacterial strains, MIC (μg/mL) of antibiotics in the absence and presence of the extract

Extract concentration

PA124

AG102

AG100Atet

EA289

CM64

KP55

KP63

NEA16

PS2636

BM47

PBSS (%)

CHL

0

128

8

128

256

256

32

32

16

64

128

 

CMI/2

32(0.25) S

< 2(< 0.25) S

64(0.5) S

256(1)I

128(0.5) S

32(1)I

32(1)I

16(1)I

16(0.25) S

128(1)I

50

CMI/4

32(0.25) S

4(0.5) S

64(0.5) S

256(1)I

128(0.5) S

32(1)I

32(1)I

16(1)I

16(0.25) S

128(1)I

50

CIP

0

64

2

> 64

> 64

> 64

2

< 0.5

4

8

< 0.5

 

CMI/2

32(0.5) S

< 0.5(< 0.25) S

> 64(≥1)I

8(> 0.125) S

32(> 0.5) S

2(1)I

< 0.5(≤1)I

< 0.5(< 0.125) S

< 0.5(< 0.062) S

< 0.5(≤1)I

60

CMI/4

32(0.5) S

< 0.5(< 0.25) S

> 64(≥1)I

16(> 0.25) S

32(> 0.5) S

2(1)I

< 0.5(≤ 1)I

< 0.5(< 0.125) S

4(0.5) S

< 0.5(≤ 1)I

60

TET

0

16

16

32

64

4

32

2

4

2

32

 

CMI/2

32(2)I

< 1(< 0.062) S

64(2)I

64(1)I

< 0.5(< 0.125) S

32(1)I

1(0.5) S

4(1)I

< 1(< 0.5) S

32(1)I

40

CMI/4

32(2)I

< 1(< 0.062) S

64(2)I

64(1)I

< 0.5(< 0.125) S

32(1)I

1(0.5) S

4(1)I

< 1(< 0.5) S

32(1)I

40

ERY

0

128

8

128

256

8

16

4

16

16

16

 

CMI/2

128(1)I

< 2(< 0.25) S

128(1)I

256(1)I

< 2(< 0,25) S

16(1)I

4(1)I

16(1)I

8(0.5) S

16(1)I

30

CMI/4

128(1)I

8(1)I

128(1)I

256(1)I

< 2(< 0,25) S

16(1)I

4(1)I

16(1)I

16(1)I

16(1)I

10

KAN

0

128

4

8

32

32

32

< 2

16

16

8

 

CMI/2

128(1)I

< 2(< 0.5) S

8(1)I

32(1)I

16(0,5) S

32(1)I

< 2(≤ 1)I

8(0.5) S

16(1)I

8(1)I

40

CMI/4

128(1)I

< 2(< 0.5) S

8(1)I

32(1)I

16(0,5) S

32(1)I

< 2(≤ 1)I

8(0.5) S

16(1)I

8(1)I

40

STR

0

256

> 256

> 256

128

8

64

8

16

> 256

64

 

CMI/2

128(0.5) S

128(≤ 0.5) S

16(≤ 0.062) S

128(1)I

< 2(< 0,25) S

64(1)I

< 2(< 0.25) S

8(0.5) S

128(≤ 0.5) S

64(1)I

70

CMI/4

128(0.5) S

256(≤ 1) S

16(≤ 0.062) S

128(1)I

< 2(< 0,25) S

64(1)I

8(1)I

8(0.5) S

> 256(≥ 1)

64(1)I

50

  1. aAntibotics [TET tetracycline, CIP ciprofloxacin, STR streptomycin, KAN kanamycin, CHL chloramphenicol, ERY erythromycin]
  2. bBacterial strains: Escherichia coli [AG102, AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [CM64, EA27, EA289], Klebsiella pneumonia [KP55, KP63], Providencia stuartii [NAE16], Enterobacter cloacae [BM47]
  3. cPBSS percentage of bacteria strain on which synergism has been observed, (): FIC (Fractional Inhibitory Concentration) of the antibiotics after association with plant extract, S Synergy, I Indifference. The values in bold represent the cases of synergy between extract and antibiotic