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Table 3 MIC of different antibiotics after the association of the crude MFS extract at MIC/2, MIC/4 against ten MDR bacteria strains

From: In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′,4′,7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria

Antibiotics Bacterial strains, MIC (μg/mL) of antibiotics in the absence and presence of the extract
Extract concentration PA124 AG102 AG100Atet EA289 CM64 KP55 KP63 NEA16 PS2636 BM47 PBSS (%)
CHL 0 128 8 128 256 256 32 32 16 64 128  
CMI/2 32(0.25) S < 2(< 0.25) S 64(0.5) S 256(1)I 128(0.5) S 32(1)I 32(1)I 16(1)I 16(0.25) S 128(1)I 50
CMI/4 32(0.25) S 4(0.5) S 64(0.5) S 256(1)I 128(0.5) S 32(1)I 32(1)I 16(1)I 16(0.25) S 128(1)I 50
CIP 0 64 2 > 64 > 64 > 64 2 < 0.5 4 8 < 0.5  
CMI/2 32(0.5) S < 0.5(< 0.25) S > 64(≥1)I 8(> 0.125) S 32(> 0.5) S 2(1)I < 0.5(≤1)I < 0.5(< 0.125) S < 0.5(< 0.062) S < 0.5(≤1)I 60
CMI/4 32(0.5) S < 0.5(< 0.25) S > 64(≥1)I 16(> 0.25) S 32(> 0.5) S 2(1)I < 0.5(≤ 1)I < 0.5(< 0.125) S 4(0.5) S < 0.5(≤ 1)I 60
TET 0 16 16 32 64 4 32 2 4 2 32  
CMI/2 32(2)I < 1(< 0.062) S 64(2)I 64(1)I < 0.5(< 0.125) S 32(1)I 1(0.5) S 4(1)I < 1(< 0.5) S 32(1)I 40
CMI/4 32(2)I < 1(< 0.062) S 64(2)I 64(1)I < 0.5(< 0.125) S 32(1)I 1(0.5) S 4(1)I < 1(< 0.5) S 32(1)I 40
ERY 0 128 8 128 256 8 16 4 16 16 16  
CMI/2 128(1)I < 2(< 0.25) S 128(1)I 256(1)I < 2(< 0,25) S 16(1)I 4(1)I 16(1)I 8(0.5) S 16(1)I 30
CMI/4 128(1)I 8(1)I 128(1)I 256(1)I < 2(< 0,25) S 16(1)I 4(1)I 16(1)I 16(1)I 16(1)I 10
KAN 0 128 4 8 32 32 32 < 2 16 16 8  
CMI/2 128(1)I < 2(< 0.5) S 8(1)I 32(1)I 16(0,5) S 32(1)I < 2(≤ 1)I 8(0.5) S 16(1)I 8(1)I 40
CMI/4 128(1)I < 2(< 0.5) S 8(1)I 32(1)I 16(0,5) S 32(1)I < 2(≤ 1)I 8(0.5) S 16(1)I 8(1)I 40
STR 0 256 > 256 > 256 128 8 64 8 16 > 256 64  
CMI/2 128(0.5) S 128(≤ 0.5) S 16(≤ 0.062) S 128(1)I < 2(< 0,25) S 64(1)I < 2(< 0.25) S 8(0.5) S 128(≤ 0.5) S 64(1)I 70
CMI/4 128(0.5) S 256(≤ 1) S 16(≤ 0.062) S 128(1)I < 2(< 0,25) S 64(1)I 8(1)I 8(0.5) S > 256(≥ 1) 64(1)I 50
  1. aAntibotics [TET tetracycline, CIP ciprofloxacin, STR streptomycin, KAN kanamycin, CHL chloramphenicol, ERY erythromycin]
  2. bBacterial strains: Escherichia coli [AG102, AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [CM64, EA27, EA289], Klebsiella pneumonia [KP55, KP63], Providencia stuartii [NAE16], Enterobacter cloacae [BM47]
  3. cPBSS percentage of bacteria strain on which synergism has been observed, (): FIC (Fractional Inhibitory Concentration) of the antibiotics after association with plant extract, S Synergy, I Indifference. The values in bold represent the cases of synergy between extract and antibiotic