In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′,4′,7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria

Dzotam, Joachim K.; Simo, Ingrid Konga; Bitchagno, Gabin; Celik, Ilhami; Sandjo, Louis P.; Tane, Pierre; Kuete, Victor

doi:10.1186/s12906-018-2084-1

BMC Complementary Medicine and Therapies

Table 3 MIC of different antibiotics after the association of the crude MFS extract at MIC/2, MIC/4 against ten MDR bacteria strains

From: In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3′,4′,7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria

Antibiotics	Bacterial strains, MIC (μg/mL) of antibiotics in the absence and presence of the extract
Antibiotics	Extract concentration	PA124	AG102	AG100Atet	EA289	CM64	KP55	KP63	NEA16	PS2636	BM47	PBSS (%)
CHL	0	128	8	128	256	256	32	32	16	64	128
	CMI/2	32(0.25) ^S	< 2(< 0.25) ^S	64(0.5) ^S	256(1)^I	128(0.5) ^S	32(1)^I	32(1)^I	16(1)^I	16(0.25) ^S	128(1)^I	50
	CMI/4	32(0.25) ^S	4(0.5) ^S	64(0.5) ^S	256(1)^I	128(0.5) ^S	32(1)^I	32(1)^I	16(1)^I	16(0.25) ^S	128(1)^I	50
CIP	0	64	2	> 64	> 64	> 64	2	< 0.5	4	8	< 0.5
	CMI/2	32(0.5) ^S	< 0.5(< 0.25) ^S	> 64(≥1)^I	8(> 0.125) ^S	32(> 0.5) ^S	2(1)^I	< 0.5(≤1)^I	< 0.5(< 0.125) ^S	< 0.5(< 0.062) ^S	< 0.5(≤1)^I	60
	CMI/4	32(0.5) ^S	< 0.5(< 0.25) ^S	> 64(≥1)^I	16(> 0.25) ^S	32(> 0.5) ^S	2(1)^I	< 0.5(≤ 1)^I	< 0.5(< 0.125) ^S	4(0.5) ^S	< 0.5(≤ 1)^I	60
TET	0	16	16	32	64	4	32	2	4	2	32
	CMI/2	32(2)^I	< 1(< 0.062) ^S	64(2)^I	64(1)^I	< 0.5(< 0.125) ^S	32(1)^I	1(0.5) ^S	4(1)^I	< 1(< 0.5) ^S	32(1)^I	40
	CMI/4	32(2)^I	< 1(< 0.062) ^S	64(2)^I	64(1)^I	< 0.5(< 0.125) ^S	32(1)^I	1(0.5) ^S	4(1)^I	< 1(< 0.5) ^S	32(1)^I	40
ERY	0	128	8	128	256	8	16	4	16	16	16
	CMI/2	128(1)^I	< 2(< 0.25) ^S	128(1)^I	256(1)^I	< 2(< 0,25) ^S	16(1)^I	4(1)^I	16(1)^I	8(0.5) ^S	16(1)^I	30
	CMI/4	128(1)^I	8(1)^I	128(1)^I	256(1)^I	< 2(< 0,25) ^S	16(1)^I	4(1)^I	16(1)^I	16(1)^I	16(1)^I	10
KAN	0	128	4	8	32	32	32	< 2	16	16	8
	CMI/2	128(1)^I	< 2(< 0.5) ^S	8(1)^I	32(1)^I	16(0,5) ^S	32(1)^I	< 2(≤ 1)^I	8(0.5) ^S	16(1)^I	8(1)^I	40
	CMI/4	128(1)^I	< 2(< 0.5) ^S	8(1)^I	32(1)^I	16(0,5) ^S	32(1)^I	< 2(≤ 1)^I	8(0.5) ^S	16(1)^I	8(1)^I	40
STR	0	256	> 256	> 256	128	8	64	8	16	> 256	64
	CMI/2	128(0.5) ^S	128(≤ 0.5) ^S	16(≤ 0.062) ^S	128(1)^I	< 2(< 0,25) ^S	64(1)^I	< 2(< 0.25) ^S	8(0.5) ^S	128(≤ 0.5) ^S	64(1)^I	70
	CMI/4	128(0.5) ^S	256(≤ 1) ^S	16(≤ 0.062) ^S	128(1)^I	< 2(< 0,25) ^S	64(1)^I	8(1)^I	8(0.5) ^S	> 256(≥ 1)	64(1)^I	50

^aAntibotics [TET tetracycline, CIP ciprofloxacin, STR streptomycin, KAN kanamycin, CHL chloramphenicol, ERY erythromycin]
^bBacterial strains: Escherichia coli [AG102, AG100Atet], Pseudomonas aeruginosa [PA124], Enterobacter aerogenes [CM64, EA27, EA289], Klebsiella pneumonia [KP55, KP63], Providencia stuartii [NAE16], Enterobacter cloacae [BM47]
^cPBSS percentage of bacteria strain on which synergism has been observed, (): FIC (Fractional Inhibitory Concentration) of the antibiotics after association with plant extract, S Synergy, I Indifference. The values in bold represent the cases of synergy between extract and antibiotic

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ISSN: 2662-7671

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