Fig. 4

MOP (a) and NOP (b) relative gene expression in SH-SY5Y neuroblastoma cells following 5 h of exposure to WSE (0.25 mg/ml, 0.50 mg/ml and 1.00 mg/ml) in association to 10 μM morphine. Data represent 2^−DDCt values calculated by using the DDCt method. Gene expression was normalized to GAPDH as housekeeping gene. Data are expressed as mean ± SEM (***p < 0.001 vs vehicle; ###p < 0.001 vs 1.00 mg/ml WSE + morphine; data analyzed by one-way ANOVA followed by Newman-Keuls tests)