Fig. 4
Effect of Rosmarinus officinalis essential oil (ROEO) on acetylcholinesterase (AChE) activity in differentiated PC12 cells. PC12 cells were seeded onto 96-well plates at a density of 1×105 cells/ml and were pre-treated with 50 ng/ml nerve growth factor (NGF) for 24 h before treatment with 10 μg/ml of ROEO for 48 h without (ROEO) or with 10 ng/ml NGF (RO + NGF). AChE activity was expressed as percentage of control. Data are presented as a percentage of the control and represent the mean of three independent trials ±.SD *P≤0.05, **P≤0.01 vs. control