HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana

Ovais, Muhammad; Ayaz, Muhammad; Khalil, Ali Talha; Shah, Sayed Afzal; Jan, Muhammad Saeed; Raza, Abida; Shahid, Muhammad; Shinwari, Zabta Khan

doi:10.1186/s12906-017-2057-9

BMC Complementary Medicine and Therapies

Table 2 HPLC-DAD identification of phenolic compounds in Olax nana leaves

From: HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana

Peak	RT (min)	Peak height (mAU)	Peak area %	Proposed identity of compound*	HPLC–DAD λmax (nm)	References
1	2.5	13.29053	0.320	Ascorbic Acid	244	Mradu et al., 2012
2	5.2	1168.32886	14.41	Gallic acid derivative	273, 279, 288	Aaby et al., 2007
3	5.3	504.33762	3.19	Gallic acid derivative	280	Mradu et al., 2012
4	5.8	456.65558	17.81	Hydroxybenzoic acid derivative	280	Santos et al., 2014
5	6.3	151.39925	8.20	Hydroxybenzoic acid derivative	274	Santos et al., 2014
6	6.9	163.31013	25.57	Gallic acid derivative	271, 278, 287	Aaby et al., 2007
7	11.1	8.41879	0.33	Kaempferol-7-O-glucoside	254	Ibrahim et al., 2015
8	11.6	26.59516	0.70	p-Coumaric acid derivative	313	Santos et al., 2014
9	13.2	35.75774	1.36	Isovitexin-4-O-glucoside	254	Ibrahim et al., 2015
10	14.3	18.4162	0.57	Caftaric acid	242; sh 298; 328	Carazzone et al., 2013
11	14.9	6.63179	0.17	Gallic acid derivative	280	Santos et al., 2014
12	15.5	19.11495	0.46	Hydroxybenzoic acid derivative	278	Santos et al., 2014
13	15.7	19.45154	0.43	Hydroxybenzoic acid derivative	278	Santos et al., 2014
14	16.9	92.68282	2.47	p-Hydroxybenzoic acid	256	Santos et al., 2014
15	17.0	15.39486	0.23	Caffeoylmalic acid	327, 300, 268	Santos et al., 2014
16	17.5	26.84283	0.60	bis-HHDP-glucose	232	Aaby et al., 2007
17	18.1	14.13607	0.21	Quercetin-3-O-triglucoside	268; 340	Lin et al., 2011
18	18.3	77.88322	1.70	Galloyl-HHDP-glucose	232	Aaby et al., 2007
19	18.9	102.11224	2.93	Apigenin-7-O-rutinoside	254	Ibrahim et al., 2015
20	19.9	19.88177	0.46	P-coumaric acid derivative	228, 316	Santos et al., 2014
21	20.7	57.97879	2.78	Vanillic acid	260; 292	Santos et al., 2014
22	21.4	10.30173	0.39	Caffeic acid	238; 298 sh; 323	Santos et al., 2014
23	22.3	89.48333	5.35	Rutin	155.3661	Reference Standard
24	23.2	4.74129	0.10	Syringic acid	274	Santos et al., 2014
25	23.5	2.06536	0.04	Proanthocyanidin trimer	284	Aaby et al., 2007
26	24.1	54.95284	1.32	Quercetin-di-glucoside	256; sh 268; 350	Llorach et al., 2008
27	24.7	38.34863	0.87	Quercetine glycoside	256; sh 266; 354	Llorach et al., 2008
28	25.4	1.90931	0.04	Quercetine glycoside	256; sh 266; 354	Llorach et al., 2008
29	25.9	11.029	0.26	Caffeoylmalic acid	244; sh 298; 328	Llorach et al., 2008
30	26.6	17.19059	0.49	p-Coumaric acid	228; 310	Santos et al., 2014
31	27.953	5.04913	0.09	Pyrogallol	244; sh 298; 328	Reference Standard
32	27.7	89.48333	2.58	Chicoric acid	244; sh 298; 328	Carazzone et al., 2013
33	29.2	8.83803	0.22	Caffeic acid derivative	242; sh 298; 322	Santos et al., 2014
34	31.3	120.93526	2.08	Quercetin-3-(caffeoyldiglucoside)- 7-glucoside	252; sh 268; 332	Santos et al., 2014
35	32.7	14.63641	0.26	Quercetin-3-d-galactoside	256; 268 sh; 356	Santos et al., 2014
36	33	14.05107	0.24	Quercetin-3-O-glucoside	256; 268 sh; 356	Santos et al., 2014
37	34	3.52791	0.06	Rosmarinic acid	330; 290 sh	Santos et al., 2014
38	36.3	2.60122	0.08	Quercetin-3-feruloylsophoroside	256; sh 268; 334	Lin et al., 2011
39	39.6	2.02323	0.06	Rosmarinic acid derivative	290; 328	Santos et al., 2014
40	40.5	2.04721	0.04	cyanidin-3-glucoside	278sh; 232	Aaby et al., 2007

*The compounds were identified by comparing absorption spectra of the sample with the standard compounds or from the values reported in the literature

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