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Table 2 HPLC-DAD identification of phenolic compounds in Olax nana leaves

From: HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana

Peak RT (min) Peak height (mAU) Peak area % Proposed identity of compound* HPLC–DAD λmax (nm) References
1 2.5 13.29053 0.320 Ascorbic Acid 244 Mradu et al., 2012
2 5.2 1168.32886 14.41 Gallic acid derivative 273, 279, 288 Aaby et al., 2007
3 5.3 504.33762 3.19 Gallic acid derivative 280 Mradu et al., 2012
4 5.8 456.65558 17.81 Hydroxybenzoic acid derivative 280 Santos et al., 2014
5 6.3 151.39925 8.20 Hydroxybenzoic acid derivative 274 Santos et al., 2014
6 6.9 163.31013 25.57 Gallic acid derivative 271, 278, 287 Aaby et al., 2007
7 11.1 8.41879 0.33 Kaempferol-7-O-glucoside 254 Ibrahim et al., 2015
8 11.6 26.59516 0.70 p-Coumaric acid derivative 313 Santos et al., 2014
9 13.2 35.75774 1.36 Isovitexin-4-O-glucoside 254 Ibrahim et al., 2015
10 14.3 18.4162 0.57 Caftaric acid 242; sh 298; 328 Carazzone et al., 2013
11 14.9 6.63179 0.17 Gallic acid derivative 280 Santos et al., 2014
12 15.5 19.11495 0.46 Hydroxybenzoic acid derivative 278 Santos et al., 2014
13 15.7 19.45154 0.43 Hydroxybenzoic acid derivative 278 Santos et al., 2014
14 16.9 92.68282 2.47 p-Hydroxybenzoic acid 256 Santos et al., 2014
15 17.0 15.39486 0.23 Caffeoylmalic acid 327, 300, 268 Santos et al., 2014
16 17.5 26.84283 0.60 bis-HHDP-glucose 232 Aaby et al., 2007
17 18.1 14.13607 0.21 Quercetin-3-O-triglucoside 268; 340 Lin et al., 2011
18 18.3 77.88322 1.70 Galloyl-HHDP-glucose 232 Aaby et al., 2007
19 18.9 102.11224 2.93 Apigenin-7-O-rutinoside 254 Ibrahim et al., 2015
20 19.9 19.88177 0.46 P-coumaric acid derivative 228, 316 Santos et al., 2014
21 20.7 57.97879 2.78 Vanillic acid 260; 292 Santos et al., 2014
22 21.4 10.30173 0.39 Caffeic acid 238; 298 sh; 323 Santos et al., 2014
23 22.3 89.48333 5.35 Rutin 155.3661 Reference Standard
24 23.2 4.74129 0.10 Syringic acid 274 Santos et al., 2014
25 23.5 2.06536 0.04 Proanthocyanidin trimer 284 Aaby et al., 2007
26 24.1 54.95284 1.32 Quercetin-di-glucoside 256; sh 268; 350 Llorach et al., 2008
27 24.7 38.34863 0.87 Quercetine glycoside 256; sh 266; 354 Llorach et al., 2008
28 25.4 1.90931 0.04 Quercetine glycoside 256; sh 266; 354 Llorach et al., 2008
29 25.9 11.029 0.26 Caffeoylmalic acid 244; sh 298; 328 Llorach et al., 2008
30 26.6 17.19059 0.49 p-Coumaric acid 228; 310 Santos et al., 2014
31 27.953 5.04913 0.09 Pyrogallol 244; sh 298; 328 Reference Standard
32 27.7 89.48333 2.58 Chicoric acid 244; sh 298; 328 Carazzone et al., 2013
33 29.2 8.83803 0.22 Caffeic acid derivative 242; sh 298; 322 Santos et al., 2014
34 31.3 120.93526 2.08 Quercetin-3-(caffeoyldiglucoside)- 7-glucoside 252; sh 268; 332 Santos et al., 2014
35 32.7 14.63641 0.26 Quercetin-3-d-galactoside 256; 268 sh; 356 Santos et al., 2014
36 33 14.05107 0.24 Quercetin-3-O-glucoside 256; 268 sh; 356 Santos et al., 2014
37 34 3.52791 0.06 Rosmarinic acid 330; 290 sh Santos et al., 2014
38 36.3 2.60122 0.08 Quercetin-3-feruloylsophoroside 256; sh 268; 334 Lin et al., 2011
39 39.6 2.02323 0.06 Rosmarinic acid derivative 290; 328 Santos et al., 2014
40 40.5 2.04721 0.04 cyanidin-3-glucoside 278sh; 232 Aaby et al., 2007
  1. *The compounds were identified by comparing absorption spectra of the sample with the standard compounds or from the values reported in the literature