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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Ethanolic extract of Streblus asper leaves protects against glutamate-induced toxicity in HT22 hippocampal neuronal cells and extends lifespan of Caenorhabditis elegans

Fig. 3

Effect of SA-EE on Nrf2 pathway activation. HT22 cells were exposed to 5 mM glutamate alone or glutamate in combination with 50 μg/mL SA-EE for 1 h prior to western blot analysis of nuclear and cytoplasmic Nrf2 levels (a). Quantitative real-time RT-PCR analysis of NQO1, GCLM and EAAT3 mRNA expression was performed after treatment of cells with 5 mM glutamate alone or glutamate in combination with 50 μg/mL SA-EE or 15 μM curcumin (positive control) for 24 h (b). Whole cell lysates were subjected to western blot analysis of EAAT3 level after treating cells with 5 mM glutamate alone or glutamate in combination with 50 μg/mL SA-EE for 24 h (c). Lamin B1 and β-actin served as endogenous loading controls for nuclear extracts and whole cell/cytoplasmic extracts, respectively. β-actin was used as an internal control for RT-PCR assay. All data were normalized to endogenous control levels and are expressed as the mean ± SEM of at least three independent experiments. ## P < 0.01, ### P < 0.001 vs. control; **P < 0.01, ***P < 0.001 vs. glutamate alone

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