Cellular pharmacodynamic effects of Pycnogenol® in patients with severe osteoarthritis: a randomized controlled pilot study

Table 1 Specific primers used in qPCR

Gene	Type	Size [bp]	Sequence [5' → 3']
GAPDH	forward	20	CGC TCT CTG CTC CTC CTG TT
GAPDH	reverse	20	CCA TGG TGT CTG AGC GAT GT
ACTB	forward	18	TGA GCG CGG CTA CAG CTT
ACTB	reverse	22	TCC TTA ATG TCA CGC ACG ATT T
18sRNA	forward	20	GCC TGC GGC TTA ATT TGA CT
18sRNA	reverse	20	ACC AGA CAA ATC GCT CCA CC
HPRT-1	forward	22	AGC CAG ACT TTG TTG GAT TTG A
HPRT-1	reverse	21	ACT GGC GAT GTC AAT AGG ACT
B2M	forward	25	AAG ATA GTT AAG TGG GAT CGA GAC A
B2M	reverse	23	AAT TCA TCC AAT CCA AAT GCG GC
SDHA	forward	22	AGA CCT AAA GCA CCT GAA GAC G
SDHA	reverse	24	CTC ATC AAT CCG ACC TTG TAG TC
MMP1	forward	20	TGG ACC TGG AGG AAA TCT TG
MMP1	reverse	20	GGT ACA TCA AAG CCC CGA TA
MMP3	forward	24	AGG CAA GAC AGC AAG GCA TAG AGA
MMP3	reverse	24	ACG CAC AGC AAC AGT AGG ATT GGA
MMP13	forward	22	TGG AAT TAA GGA GCA TGG CGA C
MMP13	reverse	20	ACC TAA GGA GTG GCC GAA CT
IL1B	forward	24	AAT CTC CGA CCA CCA CTA CAG CAA
IL1B	reverse	24	AAG GGA AAG AAG GTG CTC AGG TCA
ADAMTS5	forward	24	ACA AGA GCC TGG AAG TGA GCA AGA
ADAMTS5	reverse	24	TGA TGC CCA CAT AAA TCC TCC CGA
CTSK	forward	20	TTC CCG CAG TAA TGA CAC CC
CTSK	reverse	20	GGA ACC ACA CTG ACC CTG AT

ISSN: 2662-7671