Fig. 5
SGT-4 blocks UVB-induced ROS generation by activating antioxidant enzymes. a Cells were incubated with indicated concentration of SGT-4 for 24 h and then washed with PBS. The cells were then exposed to a UVB (100 mJ/cm2) light source for 20 min, and stained with DCF-DA for 10 min. Intracellular ROS production was analyzed using confocal microscopy. b HFFs were treated with various doses of SGT-4 for 18 h to determine the expression levels of SOD1, catalase, and GPX1 using Western blot analysis. c Human dermal fibroblasts (HDFs) were treated with various doses of SGT-4 for 18 h to determine the expression levels of GPX1 using Western blot analysis (d) HFFs were treated with indicates doses of SGT-4 for 24 h to determine the expression levels of glutathione (GSH) assay kit. e hiPSCs were incubated with indicated concentration of SGT-4 for 24 h and then ATP levels were measure by using ATP luminescent detection kit