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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula

Fig. 3

SGT-4 promotes hiPSC generation. a and b Estimated timeline of hiPSC generation and the relative number of generated AP+ colonies. After the cells were transfected with OSKM, they were maintained in HFF medium for 5 days, after, which they were treated with one of multiple doses of SGT-4 and transferred into human embryonic stem cells medium. The images highlight the comparison of the number of AP+ colonies generated from HFFs. c Immunostaining of the pluripotent-associated markers (Oct4, Nanog, and Sox-2) and counterstaining with β-actin antibodies

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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