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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula

Fig. 2

Generation and characterization of HFF-derived hiPSCs. a Typical morphology of human foreskin fibroblast (HFFs). Phase contrast, AP staining and immunostaining of the pluripotent-associated marker Oct4; Counter-staining with DAPI. b RT-PCR results demonstrated that three randomly selected 2F HFF-iPSC colonies expressed the pluripotent marker genes KIf4, c-Myc, Oct4, Nanog, and Sox2. c FASC analysis of the colonies differentiated into HFF-derived iPSCs in the present of SGT-4 on day 9 after transfection hiPSC markers including SSEA1, SSEA3, and SSEA4 antibodies

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