Fig. 3

Inhibitory effect of DHSMT on the lipid accumulation in 3T3-L1 adipocytes during adipogenesis. 3T3-L1 cells were differentiated into adipocytes by incubation with a mixture of 3-isobutyl-1-methylisobutylxanthine, dexamethasone and insulin (MDI) and exposed to various concentrations of DHSMT (0, 62.5, 125, 250 and 500 μg/ml) and SB203580 for 7 days. a Lipid accumulation in cells were evaluated by Oil red O staining and visualized using microscope at ×100 of magnification. b Stained lipid droplet was dissolved in DMSO and quantified by reading the absorbance at 530 nm. The data are mean values of three experiments ± SEM; ## < 0.01 compared with control, *P < 0.05; **P < 0.01 compared with the MDI