Fig. 4

Western blot analysis of NF-κB signal transduction in hSF and SW982 cells. The NF-κB signalling was observed l at 0, 5, 10, 15 and 30 min after the addition of TNF-α 10 ng/m to hSF or SW982 cells pre-treated with 1 μM sesamin as described in the Materials and Methods section. a Western blot results of NF-κB signalling in hSF and SW982 under TNF-α-induced conditions with or without sesamin treatment. b Graphs of phosphorylation band densities of IκB and p65 relative to the total form of each cell type. Values are presented as the mean ± SEM (n = 3). #, * =  p < 0.05; ##, ** =  p < 0.01 versus control 0 min (#) or TNF-α treatment at various time points (*) by one-way ANOVA